Phone: (608) 265-5211 | Fax: (608) 262-7306
RESEARCH INTERESTS - Regulation of terminal differentiation and functional maturation of stem and progenitor cells, regenerative biology of the endocrine pancreas, diabetes
Post doc and graduate students positions available – please contact Dr. Blum directly with CV:
B.Sc., Life Sciences, Ben-Gurion University of the Negev, Israel
M.Sc., Medical Biochemistry, The Hebrew University of Jerusalem, Israel
Ph.D., Genetics, The Hebrew University of Jerusalem
Postdoctoral Research, Stem Cell and Regenerative Biology, Harvard University
How does a newly formed stem or progenitor cell “know” it had reached its full differentiation capacity, and should assume its mature function? What part of the signal towards terminal differentiation comes from the cell’s interactions with its surrounding environment, and what is encoded in the blueprint of its intrinsic developmental program? How is this functionally mature state, once achieved, sustained throughout adult life, or tip off balance and erode in degenerative disease? And what exactly is a fully differentiated, functionally mature cell state? Not only do these questions represent fundamental problems in developmental biology, but they are also crucial in regenerative medicine, where one wants to impose a functionally mature phenotype upon stem cells differentiated in vitro, or prevent the loss of the mature cell state in degenerative diseases.
Our lab uses human pluripotent stem cells differentiation, developmental biology of the pancreas, mouse genetics, bioinformatics and functional genomic analyses to discover the genetic and molecular regulatory circuits controlling the development, maintenance, collapse and recovery of the fully differentiated, functionally mature β cell state in mice and humans.
Expanding our knowledge of functional β cell maturation will have an important impact on both stem cell based therapy for type-1 diabetes and on the prevention of irreversible β cell de-differentiation in type-2 diabetes. Specifically, it may lead to the ability to genetically set naive β cells differentiated from stem cells in vitro to produce and secrete exactly the right amount of insulin in response to a given concentration of glucose, as well as to prevent de-differentiation and maintain functional β cell maturation in diabetics.
- Adams, M. T., Gilbert, J. M., Paiz, J. H., Bowman, F. M., & Blum, B. (2018). Endocrine cell type sorting and mature architecture in the islets of Langerhans require expression of Roundabout receptors in β cells. Scientific Reports, 8(1). doi:10.1038/s41598-018-29118-x
- Gilbert, J. M., & Blum, B. (2018). Synaptotagmins Tweak Functional β Cell Maturation. Developmental Cell,45(3), 284-286. doi:10.1016/j.devcel.2018.04.018
- Blum, B., Roose, A.N., Barrandon, O., Maehr, R., Arvanites, A.C., Davidow, L.S., Davis, J.C., Peterson, Q.P., Rubin, L.L., Melton, D.A., (2014) Reversal of β cell de-differentiation by a small molecule inhibitor of the TGFβ pathway. eLife, e02809
- Blum, B., Hrvatin, S., Schuetz, C., Bonal, C., Rezania, A., Melton, D.A., (2012) Functional beta-cell maturation is marked by an increased glucose threshold and by expression of urocortin 3. Nat. Biotechnol., 30:261-264